Features and Benefits

Compatible with high-throughput handling systems.

General description

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.

Principle

Matrix metalloproteinase-3 (MMP-3) assay kit, MMP-3 hydrolyzes a specific FRET (fluorescence resonance energy transfer) substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically (lex = 325 nm/lem = 393 nm). The kit provides a rapid, simple, sensitive and reliable test, which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is ﹤50 mU.

Suitability

Suitable for measuring MMP-3 activity in various biological samples.